Abstract

The research aimed to explore the influence of new sports nutritious food (Hydrogen Water) on exercise-induced oxidative stress injury and athletic ability of power organic skeletal muscle that is susceptible to oxidation attacks and in vivo basis and possible mechanism of hydrogen water in the process of selective oxidation. This study randomly divided 80 healthy male SD rats into 8 groups of quiet control group (EC, group A), exercise control group (EE, group B), hydrogen water injection before exercise group (EH, group C) and joint hydrogen water injections before and after exercise group (EM, group D), with 10 rats in each group. The rest groups conducted adaptive low intensive treadmill running for 1 time every day before a week of the experiment except EC (group A). EH (group C) conducted intraperitoneal injection of hydrogen water with 10 mL/kg before 1 min of exercise before formal experiment. EM (group D) conducted intraperitoneal injection of hydrogen water immediately in 1 min before and after exercise. EE (group B) conducted intraperitoneal injection with same volume normal saline. EE, EH and EM group conducted a one-time heavy intensive exhausted treadmill running by using Marra project. Group B, C, D conducted quantitative treadmill running, detected 3-NT, 8-OHdG value of skeletal muscle after 3 h of exercise in group A, B, C, D using Elisa method and measured MDA, SOD, GSH, T-AOC and level of O2-, H2O2 and •OH using chemical colorimetry. The results showed that hydrogen water can significantly prolong the duration of exercise to exhaustion of rats, which had significant anti-fatigue effect; and can significantly inhibit the aggravation of motility of O2-, H2O2 and •OH, remove in vivo cytotoxic substances •OH effectively, improve SOD and GSH, enhance T-AOC, improve minicirculation and reduce the damage of cells; joint hydrogen water injections before and after exercise had synergistic effect for the prevention and control of exercise-induced oxidative stress injury. These results confirm that hydrogen water has anti-fatigue effect and protective effect of exercise-induced oxidative stress injury, which is conducted through multiple targets on the basis of selective antioxidant effect of hydrogen water.

Keywords:

Antioxidant, exercise-induced oxidative damage, hydrogen water, skeletal muscle,

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Competing interests

The authors have no competing interests.

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