Research Article | OPEN ACCESS
The Development of an Electrochemical Immunosensory for 1-naphthylacetic Acid
S.Z. Liang, J.R. Pan, X.M. Yin and K. Sui
College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang Province, 310018, China
Advance Journal of Food Science and Technology 2015 6:439-443
Received: March 17, 2015 | Accepted: March 24, 2015 | Published: August 25, 2015
Abstract
A new detection method for 1-naphthylacetic acid (NAA) based on the electrochemical immunosensor was introduced in the study, in which anti-NAA polyclonal antibody was used. An monolayer-molecular, self-reassemble membrane was made from 3-mercaptopropionic acid on the surface of gold electrodes, on the membrane the anti-NAA antibody was immoblize through ester-activated method with addition of 1-(3-Dimethylaminopropyl)-3- ethylcarbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS). Based on the three-electrode system, an NAA immunosenory detection method was established with using the signal from cyclic voltammetry. The standard curve could be represented as △E = 0.0407lgc+0.0701(R2 = 0.9984) with the range from 0.05 μg/mL to 2 μg/mL and with the detection limit was 0.035 μg/mL. The sensory method had very low cross-reactivity, lower than 3%, to NAA similarities such as phenylacetic acid and indolyl-3-acetic acid. In addition, the sensor could be used with high stability as long as 30 days (room temperature). It indicated that a specific, stable electrochemical immunosensory detection method for 1-Naphthaleneacetic acid was successfully developed.
Keywords:
1-naphthylacetic acid, electrochemical immunosensor, self-assembled monolayer membrance,
References
-
Alberto, N., B. Rosario and L.V. José, 1997. Determination of 1-naphthylacetic acid in commercial formulations and natural waters by solid-phase spectrofluorimetry. Microchim. Acta, 126(1-2): 33-38.
CrossRef Direct Link
-
Chen, H.P., X. Liu, C.P. Wang and P. Yin, 2012. Determination of gibberellic acid and a-naphthalene acetic acid in tea by ultra-pressure liquid chromatography tandem mass spectrometry with dispersive solid phase extraction. Chinese J. Anal. Chem., 40(7): 1059-1064.
-
Chen, X.D., Q.M. Lu, J.T. Cheng and L. Zhang, 2015. Determination of plant hormones by capillary electrophoresis based on polymer micelles. Chinese J. Anal. Chem., 43(1): 127-131.
-
Dong, S.L., L.M. Du, Q.Q. Xu and Y.X. Gao, 2002. Simultaneous determination of a-naphthylacetic acid and indole-3-acetic acid by derivation synchronous fluorimetry. J. Anal. Sci., 18(1): 33-35.
-
Esparza, X., E. Moyano, J.R. Cosialls and M.T. Galceran, 2013. Determination of naphthalene-derived compounds in apples by ultra-high performance liquid chromatography-tandem mass spectrometry. Anal. Chim. Acta, 782C: 28-36.
CrossRef PMid:23708281 Direct Link
-
Gao, X.Y., S. Wang, S. Chen and Y.R. Zhu, 2013. Determination of a-naphthaleneacetic acid in vegetables and fruits by flow injection chemiluminescence with aqueous two-phase extraction. Food Sci., 34(20): 213-216.
Direct Link
-
George, S.A. Jr., 1942. Control of growth and differentiation in plants. Cold Spring Harb. Sym., 10: 1-6.
CrossRef Direct Link
-
Guan, W.B., P.J. Xu, K. Wang, Y. Song and H.Y. Zhang, 2011. Determination and study on dissipation of 1-naphthylacetic acid in garlic and soil using high performance liquid chromatography-tandem mass spectrometry. Food Chem. Toxicol., 49(11): 2869-2874.
CrossRef PMid:21864633 Direct Link
-
Halamek, J., M. Hepel and P. Skladal, 2001. Investigation of highly sensitive piezoelectric immunosensors for 2,4-dichlorophenoxyacetic acid. Biosens. Bioelectron., 16(4-5): 253-260.
CrossRef Direct Link
-
Jose, A.M.P., F.C.B. Luisa, S.F.R. Ignacio and B.R. Sonia, 2012. Simultaneous determination of plant growth regulators 1-naphthylacetic acid and 2-naphthoxyacetic acid in fruit and vegetable samples by room temperature phosphorescence. Phytochem. Analysis, 23(3): 214-221.
CrossRef PMid:21805518 Direct Link
-
Keay, R.W. and C.J. McNeil, 1998. Separation-free electrochemical immunosensor for rapid determination of atrazine. Biosens. Bioelectron., 12(9): 963-970.
CrossRef Direct Link
-
Kong, D.Y., L.L. Shi, Z.J. Shan, F. Ge and S.X. Gao, 2010. Determination of a-naphthylacetic acid by ASE-GPC coupled with HPLC. J. Instrum. Anal., 29(4): 382-385.
Direct Link
-
Liang, H.L., H.N. Liu, Q.H. Yang, R.Z. Huang, X. Wei, W.H. Ye, W.H. Luo and Z.C. Xiong, 2013. Seed germination of Rhodomyrtus tomentosa. Seed Sci. Technol., 41(2): 188-198.
CrossRef Direct Link
-
Limbut, W., P. Kanatharana, B. Mattiasson, P. Asawatreranakul and P. Thavarungkul, 2006. A comparative study of capacitive immunosensors based on self-assembled monolayers formed from thiourea, thioctic acid,and 3-mercaptopropionic acid. Biosens. Bioelectron., 22(2): 233-240.
CrossRef PMid:16460923 Direct Link
-
Liu, H.T., Y.F. Li, T.G. Luan, C.Y. Lan and W.S. Shu, 2007. Simultaneous determination of phytohormones in plant extracts using SPME and HPLC. Chromatographia, 66(7-8): 515-520.
CrossRef Direct Link
-
Mohamed, B., B.R. Karima, C. Amani, M. Benchir, B. Amina, M. Abderrazak and J.R. Nicole, 2012. An electrochemical immunosensor for detection of Staphylococcus aureus bacteria based on immobilization of antibodies on self-assembled monolayers-functionalized gold electrode. Biosensors, 2(4): 417-426.
CrossRef PMid:25586032 PMCid:PMC4263564 Direct Link
-
Nagayama, T., I. Takano, M. Kobayashi, Y. Tamura, S. Tomizawa, Y. Tateishi, N. Kimura, K. Kitayama and K. Saito, 2003. Determination of captafol, cyhexatin, 1-naphthylacetic acid and quintozene in apple, Japanese pear and melon by simultaneous extraction. Shokuhin Eiseigaku Zaashi, 44(2): 126-131.
CrossRef PMid:12846161 Direct Link
-
Robert, C.L., 1939. Effects of indoleacetic and naphthylacetic acids on development of buds and roots in horseradish. Bot. Gaz., 100(3): 500-527.
CrossRef Direct Link
-
Sun, B., L.J. Chen, Y. Xu, M. Liu, H.S. Yin and S.Y. Ai, 2014. Ultrasensitive photoelectrochemical immunoassay of indole-3-acetic acid based on the MPA modified CdS/RGO nanocomposites decorated ITO electrode. Biosens. Bioelectron., 51: 164-169.
CrossRef PMid:23954674 Direct Link
-
Sun, X., S.Y. Du, X.Y. Wang, W.P. Zhao and Q.Q. Li, 2011. A label-free electrochemical immunosensor for carbofuran detection based on a sol-gel entrapped antibody. Sensors (Basel), 11(10): 9520-9531.
CrossRef PMid:22163709 PMCid:PMC3231269 Direct Link
-
Tang, D.P., R. Yuan, Y.Q. Chai, Y. Liu, X. Zhong and J.Y. Dai, 2004. Preparation and application of a potentiometric platinum immunosensor based on colloidal gold and polyvinyl butyral. J. Southwest China Norm. Univ., Nat. Sci. Edn., 29(5): 843-847.
-
Wang, D.X., Y.M. Zhou and W. Chen, 2003. Direct determination of naphthylacetic acid by GC with sol-gel capillary column. Anal. Instrum., 3: 35-37.
Direct Link
-
Wang, M.Y., J.R. Huang, M. Wang, D.G. Zhang and J. Chen, 2014. Electrochemical nonenzymatic sensor based on CoO decorated reduced graphene oxide for the simultaneous determination of carbofuran and carbaryl in fruits and vegetables. Food Chem., 151: 191-197.
CrossRef PMid:24423520 Direct Link
-
Wu, P., Y. Tan, J. Zhang, L.Y. Wang, J. Tang, W. Jiang, X.D. Pan, B.J. Ma, Z.N. Ni and T.J. Wang, 2014. Determination of 10 plant growth regulators in bean sprouts by sequential cleaning-gas chromatography-mass spectrometry. Chinese J. Anal. Chem., 42(6): 866-871.
Direct Link
-
Xu, W.T. and R.H. Zhu, 2009. Rapid simultaneous analysis of a-naphthylacetic acid and 6-benzylaminopurine by synchronous-derivative solid-substrate room temperature phosphorimetry. Chinese J. Anal. Lab., 28(3): 99-102.
Direct Link
-
Xue, X.M., C. Lu, P.X. Nie, G.L. Wang and J.Z. Wang, 2012. Research advances in chemical thinning of flowers and fruits in fruit tree. Acta Agr. Jiangxi, 24(2): 52-57.
Direct Link
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
|
|
![](http://www.maxwellsci.com/images/AJFST-maxw.jpg) |
ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
![Submit Manuscript](../images/sub.jpg) |
Information |
|
|
|
Sales & Services |
|
|
|