Abstract

Extraction process was optimized by single factor and orthogonal experiment $(L_9) (3^4)$. Moreover, the content determination was studied in methodology. The optimum ultrasonic extraction conditions were: ethanol concentration of 75%, ultrasonic power of 420 w, the solid-liquid ratio of 1:15, extraction duration of 45 min, extraction temperature of 90°C and extraction for 2 times. Saponins content in Guazhou samples was significantly higher than those in Xinjiang and Inner Mongolia. Meanwhile, Guazhou samples harvested in April and May were higher than that in Guazhou Cynomorium songaricum numbered “the three-nine”. In addition, saponin content in various samples grown in separate years is different. This ultrasonic extraction process can significantly improve the saponins extraction efficiency. Determination by this method is fast, easy-to-operate. The result of this method is reliable. The conclusion of study could provide scientific basis for rational development and quality control of Cynomorium songaricum Rupr.

Keywords:

Content determination, Cynomorium songaricum Rupr, process optimization, saponins, ultrasonic extraction,

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Competing interests

The authors have no competing interests.

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