Research Article | OPEN ACCESS
The Preparation of Bioimprinted Whole-cell Biocatalyst and Its Application in Bioconversion of Biodiesel
1, 2Meiling Chen, 2, 3Qingqing Li, 2Hui Ruan and 2Guoqing He
1School of Food Science and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China
2School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China
3Zhejiang Academy of medical sciences, Hangzhou 310007, China
Advance Journal of Food Science and Technology 2015 3:227-232
Received: March ‎3, ‎2015 | Accepted: March ‎14, ‎2015 | Published: August 10, 2015
Abstract
Biodiesel has attracted considerable attention as an environmentally friendly alternative fuel. Lipase is the most popular enzyme for biodiesel production and immobilization has been deployed to improve enzyme stability and reusability. Exploitation of high activity lipase is the key point for biodiesel production. Whole-cell biocatalysts have been applied in the biosynthesis of biodiesel and bioimprinting is a promising approach for enzyme performances improvement. In this study, based on the S. cerevisiae cell-surface display system with $\alpha$-agglutinin as anchor, a whole-cell biocatalyst of codon-optimized Rhizopus oryzae lipase was constructed and bioimprinted with oleic acid, gaining 5-fold increase on enzymatic activity in the alcoholysis of soybean oil to biodiesel. Moreover, the conversion of FAME was up to 95.45±2.73% after a 27-h reaction at 60°C. Our results indicated that combining bioimprinting with yeast display technique to prepare whole-cell biocatalyst could result in potential enzymes for bioconversion of biodiesel in organic solvents.
Keywords:
Biodiesel, bioimprinting, Rhizopus oryzae Lipase (ROL), whole-cell biocatalyst, yeast surface display,
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Competing interests
The authors have no competing interests.
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This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
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