Research Article | OPEN ACCESS
Frequency of Methicillin-resistant Staphylococcus aureus isolates from Clinical Specimens in Gondar University Hospital, Northwest Ethiopia
1Belay Anagaw, 1Yitayal Shiferaw, 2Berhanu Anagaw, 3, 4Fantahun Biadglegne, 1Feleke Moges, 1Afework Kassu, 1Chandrashekhar Unakal and 1, 5Andargachew Mulu
1Department of Medical Microbiology, College of Medicine and Health Sciences,
University of Gondar, P.O. Box 196, Gondar, Ethiopia
2Faculty of Sciences, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia
3Faculty of Medicine and Health Sciences, Bahir Dar University, P.O. Box 79, Bahir Dar, Ethiopia
4Institute of Medical Microbiology and Epidemiology of Infectious Diseases, Faculty of Medicine, University of Leipzig, Leipzig, Germany
5Institute of Virology, Faculty of Medicine, University of Leipzig, Germany
Asian Journal of Medical Sciences 2013 3:59-64
Received: December 26, 2012 | Accepted: February 01, 2013 | Published: June 25, 2013
Abstract
The development of resistance to multiple antibiotics and control of disease transmission by MRSA isolates in hospitals/communities have been recognized as the major challenges as the bacterial population that expresses the resistance phenotype varies according to the environmental conditions. This study was conducted to determine the magnitude of MRSA strain and to investigate the in vitro antimicrobial susceptibility pattern and &beta-lactamase production of strains isolated from clinical specimens. Total of 1,295 clinical specimens including: pus, wound swab and discharge and body fluids were collected from patients presenting with infection. The presence of Staphylococcus aureus was detected using conventional microbiological methods. Isolated S. aureus were further subjected to MRSA screening and subsequently the antibiotic susceptibility test was performed. The data were entered and analyzed using SPSS. Of the total 279 S. aureus isolates during the study period (21.5%), 49 (17.6%) were found to be MRSA. Most of MRSA were isolated from wound swab and discharge and from inpatient. All MRSA strains recorded susceptibility to vancomycin, flucloxacillin, cefadroxil and cefoxitin, which was followed by 95.9% to clindamycin. In contrast, all strains of MRSA were found to be resistance to penicillin and 78.7% of them were found to be multidrug resistant. Both &beta-lactamase productions were detected in all S. auresus irrespective of methicillin-resistant. According to this study, vancomycin, flucloxacillin, cefadroxil and cefoxitin seems to be most effective antimicrobial agents which shows 100% sensitivity even with multi-drug resistance.
Keywords:
Clinical specimens, frequency, gondar ethiopia, MRSA, Staphylococcus aureus,
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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ISSN (Online): 2040-8773
ISSN (Print): 2040-8765 |
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