Research Article | OPEN ACCESS
Effect of Long-term use of Sida rhombifolia L. Extract on Haemato-biochemical Parameters of Experimental Animals
1M. Ouedraogo, 2P. Zerbo, 3K. Konate, 4N. Barro and 1Laya L. Sawadogo
1Laboratory of Animal Physiology, University of Ouagadougou, 09 P.O. Box: 848,
Ouagadougou 09, Burkina Faso
2Laboratory of Plants Ecology and Biology, University of Ouagadougou,
09 P.O. Box: 848, Ouagadougou 09, Burkina Faso
3Laboratory of Biochemistry and Applied Chemistry, University of Ouagadougou,
09 P.O. Box: 848, Ouagadougou 09, Burkina Faso
4Laboratory of Biochemistry and Molecular Genetics Microbial, University of
Ouagadougou, 03 P.O. Box: 7131, Ouagadougou 03, Burkina Faso
British Journal of Pharmacology and Toxicology 2013 1:18-24
Received: December 3, 2012 | Accepted: January 01, 2013 | Published: February 25, 2013
Abstract
Sida rhombifolia L. is used as a remedy for inflammatory disorde and some other diseases in African folk medicine. The present study was conducted to evaluate the haematological and biochemical parameters and toxic effects of aqueous acetone extracts of Sida rhombifolia L. in mice Swiss and albinos Wistar rats. Sida rhombifolia L. is used as a remedy for inflammatory disorde and some other diseases in African folk medicine. In an acute toxicity study, Sida rhombifolia extract was administered orally at doses ranging between 1-6 g/kg to experimental mice and observed for any toxic symptoms up to 14 days. In sub-acute toxicity, Sida rhombifolia extract was tested at dose levels of 100, 200 and 300 mg/kg on physical (body weight and organ weight), biochemical and haemtological parameters in adult albino rats. The Sida rhombifolia extract was well tolerated at the acute administration. No mortality was observed even at the highest dose of 6 g/kg (the value of LD50 is greater than 5000 mg/kg). In the sub-acute administration, Sida rhombifolia extract significantly (p<0.05) prevented an increase in body weight in a dose dependent manner and this reflected a significant (p<0.05) increase in the relative organs (liver and heart) weights. The biochemical parameters showed a significant (p<0.05) increase in ALT, AST and ALP. However for the other biochemical parameters (glucose, creatinine, urea nitrogen, triglycerides, total bilirubin and direct bilirubin), there is a significant decrease between the control group (10% DMSO) and the other treated groups (p<0.05 and p<0.01). The insipient toxicological effect is more significant in the biochemical rather than in the haematopoietic system of rats. There is a significant decrease for monocytes, basophils, haemoglobin, haematocrit and MCV between the control group (10% DMSO) and the treated groups (p<0.05). At last, one could say that the reported pharmacological and therapeutic effectiveness of Sida rhombifolia extract is not without toxicity implication.
Keywords:
Biological parameters, LD50, mice, Sida rhombifolia L, toxicity, wistar rats,
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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