Research Article | OPEN ACCESS
The Effect of Exhaust Fumes on Glutathione S-Transferase Enzymes in the Lung of Rats Supplemented with Natural Products
1Muhammad Y. Gwarzo and 2Fredrick O. Ujah
1Department of Chemical Pathology, Faculty of Medicine, Bayero University Kano,
PMB 3011, Kano, Nigeria
2Department of Biochemistry, Faculty of Science, Bayero University, Kano, PMB 3011, Kano Nigeria
British Journal of Pharmacology and Toxicology 2013 4:136-141
Received: November 08, 2012 | Accepted: February 08, 2013 | Published: August 25, 2013
Abstract
This study examined the effect of exhaust fumes on the lungs and the impact of dietary supplementation with natural products containing cancer chemopreventive agents in attenuating their effect. Thirty-two rats were grouped into eight groups of four rats each. Groups 1-3 were on non-supplemented diet and exposed to exhaust fumes from generator for time intervals of 5 mins, 1 h and 2 h, respectively at a distance of 2.5 m away from the generator. Groups 4-6 were fed on supplemented diet and exposed to exhaust fumes for time intervals of 5 min, 1h and 2 h, respectively at a distance of 2.5 m from the generator. Group 7 was positive control not exposed to exhausted fumes and fed on diet supplemented with natural products. Group 8 was positive control not exposed to exhaust fumes and not on supplement diet. Normal cellular architecture was observed in supplement positive control groups compared with non supplement positive control groups indicated that the integrity of tissues were not compromised following food supplementation. However, large deposit of dark spots were seen in lungs of non supplemented groups on 1h and 2 h exposure groups, respectively. The lungs also showed significant decrease in the Glutathione-S-Transferase (GST) level on exposure for 5 min, 1hour and 2 h (p<0.05) compared with their respective control groups. It was also observed that the level of Malondialdehyde (MDA) increased significantly (p<0.05) in non supplement groups compared with their control groups. Combination of natural products significantly reversed the effect of exhaust fumes on the level of GST (p<0.05) and MDA level (p>0.05 compared with non supplement groups. Supplementation of diet with natural products had no adverse effect on the integrity of the tissues under examination as demonstrated by histochemical analysis. Hence, combination of natural diet may provide a useful preventive measure against tissue injury consequent to exposed to exhaust fumes experienced in our houses and roads.
Keywords:
Chemo preventive agents, carcinogenic, neurotoxic, endogenous antioxidant enzyme,
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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ISSN (Online): 2044-2467
ISSN (Print): 2044-2459 |
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