Research Article | OPEN ACCESS
Effect of Plant Extract Eugenia caryophyllus, Cinnamon zeylanicum on Antibiotic resistant from Staphylococcus aureus
Sabiha Sharif Salih, Nask Muhammad Faraj and Abdul Mostafa Hamarash
Technical Agricultural Institute, Sulaymani, Iraq
British Journal of Pharmacology and Toxicology 2014 4:133-136
Received: January 21, 2014 | Accepted: March 08, 2014 | Published: August 20, 2014
Abstract
The aim of this study was determination antimicrobial activity of ethanol extract and Watery of Eugenia caryophyllus, Cinnamon zeylanicum effect resistant genes in Staphylococcus aureus from Tomato in Green house, market in Sulaymani city-Iraq. The isolates were identified through cultural, morphological and biochemical examination, in addition to API Staph system. Susceptibility test to ten antimicrobials were performed for all isolates. The isolates were grouped to 7 antibiogram; The S. aureus isolates were 100% resist to Amp, Cef, Ax, Gm, Tet and Tri. Some isolated were resistant to at least one more antibiotics. The Sub Minimum Inhibition Concentration (SMIC) of ethanol extract and watery for Eugenia caryophyllus determined (2000 μg/mL), S MIC for Cinnamon (2500 μg/mL). Treating S. aureus S1, S3 by both medicinal plant, the results showed that Clove extract, appeared high inhibitory effect on the growth of' 'Staphylococcus aureus, this inhibition converted some resistance genes in S1, S2 to sensitive.
Keywords:
Antimicrobial, cinnamon ethanol extract, Eugenia caryophyllus, Staphylococcus aureus, watery extract,
References
-
Adel, K.K. and S.S. Sabiha, 2010. Genetic site determination of antibiotic resistance genes in Pseudomonas aeruginosa by genetic transformation. Brit. J. Pharmacol. Toxicol., 1(2): 85-89.
-
Ali, S.M., A.A. Khan, I. Ahmed, M. Musaddiq, K.S. Ahmed, H. Polasa et al., 2005. Antimicrobial activities of Eugenol and Cinnamaldehyde against the human gastric pathogen Helicobacter pylori. Ann. Clin. Microbiol. Antimicrob., 4: 20.
CrossRef PMid:16371157 PMCid:PMC1373661 -
Andrews W.H. and T. Hammack, 2000. Bacteriological Analytical Manual. US Food and Drug Administration, FDA.
-
Atlas, R.M., A.E. Brown and L.C. Parks, 1995. Laboratory Manual Experimental Microbiology. Mosby-year Book, Inc., St. Louis.
PMCid:PMC167375 -
Cowan, M.M., 1999. Plant products as antimicrobial agents. Clin. Microbiol. Rev., 12: 564-582.
CrossRef PMid:10515903 PMCid:PMC88925 -
Cruickshank, R., J.D. Duguid, B.P. Marmion and R.H.A. Swain, 1975. Medical Microbiology the Practice of Medical Microbiology. 12th Edn., Vol. 1, Churchill Livingstone, London.
PMid:10246390 -
Emori, T.G. and R.P. Gaynes, 1993. An overview of nosocomial infections, including the role of the microbiology laboratory. Clin. Microbiol. Rev., 6: 428-442.
CrossRef PMid:8269394 PMCid:PMC358296 -
Hamilton-Miller, J.M.T., 1995. Antimicrobial properties of tea (Camellia sinensis L.). Antimicrob. Agents Ch., 39(11): 2375-2377.
CrossRef -
Hernandez-Brenes, C., 2002a. The importance of training for improving the safety and quality of fresh fruits and vegetables. In: Improving the safety and quality of fresh fruits and vegetables: A training manual for trainers. University of Maryland.
-
IIcim, A. and M. Digrak, 1998. The investigation of antimicrobial effect of some plant extract. J. Biol., 22: 1.
-
Kathe, W., S. Honnef and A. Heym, 2003. Medical and Aromatic Plant in Albania. Bosnia-Herzegovnia, Bulgaria, Croatia and Romania: A study of the collection of and trade in Medicinal and Aromatic Plants (MAPs), relevant legislation and the potential of MAP use for financing nature conservation and protected areas. Federal Agency for Nature Conservation, Bonn. BfN-Skripten no. 91.
PMid:12713806 -
Life Science Journal, 2009. 6(3): 80- 82, ISSN: 1097-8135.
-
McClure, J.W., 1975. Physiology and Function of Flavonoids. In: Harborne, J.B., T.J. Mabry and H. Mabry (Eds.), the Flavonoids. Academic Press, New York, pp: 970-1055.
CrossRef -
Nascimento, G.G.F., J. Lacatelli, P.C. Freitas and G.L. Silva, 2000. Antibacteria activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Braz. J. Microbiol., 31(4): 886-891.
CrossRef -
Ranjan, S., N. Dasgupta, P. Saha, M. Rakshit and C. Ramalingam, 2012. Comparative study of antibacterial activity of garlic and cinnamon at different temperature and its application on preservation of fish. Adv. Appl. Sci. Res., 3(1): 495-501.
-
Toda, M., S. Okubo, R. Hiyoshi and T. Shimamura, 1989. The bacterial activity of rea and coffee. Lett. Appl. Microbiol., 8: 123-125.
CrossRef -
Vainker, S., 2003. Southeast Asian exports since the 14th century: Cloves, pepper, coffee. J. Early Modern History, 6: 296-307.
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
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The authors have no competing interests.
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