Abstract

This study concerned with the isolation of Pseudomonas aeruginosa from various clinical cases in human which include (burn, wound and urine) that admitted to Emergency hospital and internal lab of teaching hospital in Erbil city. Forty isolates of P. aeruginosa from out of 120 samples were identified by using cultured, morphological and biochemical tests in addition to vitek machine. According to the resistance of the isolates to these antibiotics they showed variation in their resistance; the highest resistance was for penicillin G with 85% while the lowest resistance was for ceftizoxime with 40% and for others ranged between 42.5-82.5%. On the other hand the isolates P9, P16 and P30 were resist to all antibiotics under study. RCR was used to detect Exotoxin A (ETA) structural gene sequence. The result revealed that 82.5% of the isolates were positive for ETA gene. Methanolic and aqueous crude extract of Kalanchoe blossfeldiana were used as medicinal plant for curing agent for the reduction of antibiotic resistance genes of P. aeruginosa isolate P9, this was done through determination of Minimum Inhibitory Concentration (MIC) of this medicinal plant that used as curing agent which was 1800 &mug/mL for aqueous extract and 1400 &mug/mL for methanolic extract. The effect of MIC of methanolic extract on antibiotic resistance genes for isolate P9 showed reduction of the resistance from 44-100% while the effect of MIC of aqueous extract on antibiotic resistance genes for isolate P9 revealed decreasing of the resistance from 36-86%, respectively.

Keywords:

Antobiotic resistance, , Kalanchoe blossfeldiana, , plasmid curing, , Pseudomonas aeruginosa,

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