Research Article | OPEN ACCESS
Beneficial Effects of Hesperidin against Cisplatin-Induced Nephrotoxicity and Oxidative Stress in Rats
1Wafaa R. Mohamed, 1El-Shaimaa A. Arafa, 1Basim A. Shehata, 3Gamal A. El Sherbiny and 2Abdel Nasser A.M. Elgendy
1Department of Pharmacology and Toxicology, Faculty of Pharmacy, Beni-Suef University
2Department of Pharmacology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511
3Department of Pharmacology and Toxicology, Faculty of Pharmacy, Kafr El-Sheikh University, Egypt
British Journal of Pharmacology and Toxicology 2015 3:56-63
Received: November 30, 2014 | Accepted: February 8, 2015 | Published: July 20, 2015
Abstract
Cisplatin has been frequently used for treatment of wide variety of tumors. The use of cisplatin is associated with severe cytotoxicity such as nephrotoxicity, hepatotoxicity and spermiotoxicity which radically limits its clinical use. The present study aimed to investigate the possible protective effects of multiple doses of hesperidin against cisplatin-induced nephrotoxicity induced by single i.p injection of cisplatin (7.5 mg/kg). Hesperidin was given to rats at two different doses (100 and 200 mg/kg p.o) for 7 days starting one day before cisplatin injection. Blood samples were collected for determination of serum creatinine and Blood Urea Nitrogen (BUN) levels. Kidneys were used for the determination of Malondialdehyde (MDA), Glutathione (GSH) and total nitrate and nitrite contents. Liver samples were also used for histopathological examination. Results showed that hesperidin significantly reduced cisplatin-induced elevations in serum creatinine and BUN levels. It also significantly reduced kidney MDA and NO content and elevated GSH content. In conclusion, hesperidin greatly protected kidney against cisplatin-induced toxicity in a dose-dependent manner.
Keywords:
Blood urea nitrogen, cisplatin, creatinine, hesperidin, nephrotoxicity, oxidative stress,
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