Abstract

Hepatotoxicity is now receiving a supreme importance. LPS is the most potent activator of macrophages. The aim of this study is to demonstrate the prophylactic activity of silimarin and curcumin against LPS-induced hepatotoxicity in rats. The liver slices had been divided into 4 groups; normal, control (LPS), LPS+silimarin and LPS+curcumin (N = 12). After 24 h, analysis of ALT, GSH, TNF-&alpha and IL-10 was carried out. ALT in the control group showed a high significant elevation in comparison to the normal group, while group 3 and 4 showed a high significant decrease compared with the control group. In control (LPS) group a significant reduction in GSH level was observed; where in group 3 and 4 a significant increase was noticed in comparison to the control group. Control (LPS) group showed a high significant elevation in TNF-&alpha level in comparison to normal group, where in group 3 and 4 a high significant decrease was detected in comparison to the control group. Level of IL-10 in the control group indicated a high significant elevation compared to the normal group, while in group 3 and 4 a high significant decrease in the IL-10 levels were detected in comparison to the control group. In conclusion, silimarin and curcumin showed a hepatoprotective activity against hepatotoxicity induced by LPS.

Keywords:

Alanine amino Transferase (ALT), Curcumin, Precision Cut Liver Slices (PCLS), hepatotoxicity, Lipopolysaccharide (LPS), prophylaxis, reduced glutathione (GSH), silymarin, Tumor Necrosis Factor alpha (TNF-&alpha) lactate dehydrogenase (IL-10),

References

Competing interests

The authors have no competing interests.

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The authors have no competing interests.