Abstract

This study aimed to develop molecular marker (s) associated with salt tolerance in barley using Inter Simple Sequence Repeat (ISSR) and Simple Sequence Repeat (SSR) markers and usefulness of both markers, to detect possible specific markers to be utilized in the barley future breeding programs for salt tolerance. From filed evaluation, genotypes Giza 123, Rehan-03 and Saiko were found to be salt tolerant while Beecher, Line 1 and line 2 were sensitive. Using 10 ISSR primers low resolution to distinguish the two barley groups was found. However, SSR primer HVM09 exhibited a band with molecular size of 125 bp which could be considered a positive molecular marker associated with salt tolerance. Based on phylogenic tree using rooted Neighbor Joining (NJ) the dendrograms constructed with SSR marker revealed two main genetic clusters, the first include the all tolerant genotypes in and were found in closely related; since, Giza 123 have similarity 89% with Rehan-03. On the other hand, second cluster contains the sensitive genotypes, similarity value 84% was recorded between line 1 and line 2 which indicated that these three genotypes were closely related to each other and this is reflected from their response to salt stress.

Keywords:

Hordeum vulgare, ISSR, molecular markers, salt stress, SSR,

References

Competing interests

The authors have no competing interests.

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Copyright

The authors have no competing interests.