Research Article | OPEN ACCESS
Identification of Mycobacterium bovis in Fresh Cheeses Expended at Markets in the Veracruz-Boca Del RÃo Metropolitan Area, Mexico
1David I. Martinez-Herrera, 1Violeta T. Pardio-Sedas, 1Juan V. Ornelas-Cruz, 1Dora Romero-Salas, 1Jose A. Villagomez-Cortes, 1Mayra Villanueva-Valencia, 2Aurora Parissi-Crivelli, 3Francisco Morales-Alvarez and 3Ricardo Flores-Castro
1Facultad de Medicina Veterinaria y Zootecnia, Circunvalacion Esq. Yanez S/N, Col. Unidad Veracruzana, C.P. 91710, Veracruz, Veracruz, Mexico
2Laboratorio Estatal de Salud Pública de Veracruz. Eucalipto S/N Lote 7 Mza. IZC., Cd. Industrial Bruno Pagliai, C.P. 91697, Veracruz, Veracruz, México
3Instituto Nacional de Investigaciones Forestales AgrÃcolas y Pecuarias, Centro Nacional de Investigación Disciplinaria en MicrobiologÃa Animal. Km 15.5 Carretera México-Toluca, Col. Palo Alto, C.P. 05110, Cuajimalpa, D.F., México
Current Research Journal of Biological Sciences 2013 3:96-103
Received: September 28, 2012 | Accepted: November 23, 2012 | Published: May 20, 2013
Abstract
The aim of this study was to identify Mycobacterium bovis presence in fresh cheese sold at markets in the Veracruz-Boca del Rio metropolitan area, Mexico and to determine the associated risk factors, using a cross-sectional study. A sample size of 30 was calculated with the program Win Episcope Ver 2.0. Samples were collected in two occasions, during the dry and rainy seasons of 2010. Sampling was conducted in six cheese markets officially registered by the health authorities. Samples were processed by conventional bacteriological and molecular techniques (PCR). Mycobacterium spp. was isolated from one of the samples (1.67%), but its typing by biochemical tests was unsuccessful; however, by PCR it was identified as M. fortuitum. There were no differences between the variables (p>0.05) and no risk factors were identified. We concluded that Mycobacterium spp. was isolated, even though it was not identified by PCR as M. bovis, but as M. fortuitum.
Keywords:
Markets, PCR, quality assurance, retailing, risk factors,
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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