Research Article | OPEN ACCESS
Screening of Antibacterial Activities of Marine Gastropod Hemifusus Pugilinus
S. Sugesh, P. Mayavu, P. Ezhilarasan, P. Sivashankar and N. Arivuselvan
CAS in Marine Biology, Annamalai University, Parangipettai-608502, Tamil Nadu, India
Current Research Journal of Biological Sciences 2013 2:49-52
Received: July 31, 2012 | Accepted: September 17, 2012 | Published: March 20, 2013
Abstract
In the present investigation was carried out to screen the antibacterial activities of marine gastropod Hemifusus pugilinus. The whole body of the animal was extracted in three different solvents such as, ethanol, methanol and water. The antibacterial properties were studied using 10 human pathogenic microorganisms such as, Escherichia coli , Klebsiella oxytoca , K. pnuemoniae, Lactobacillus vulgaris , Proteus mirabilis, Pseudomonas aeruginosa, Salmonella typhi, S. paratyphi , Staphylococcus aureus and Vibrio paraheamolyticus. The ethanolic extracts of H. pugilinus showed maximum antibacterial activities against E. coli (8 mm) and minimum activities against Vibrio paraheamolyticus (2 mm), Methanolic extracts showed highest activity in E. coli (6 mm) and lowest activity against S. paratyphi (1 mm) and the extract of water showed antibacterial activities against E. coli , K. oxytoca and S. paratyphi . The crude extracts were purified in silica gel column chromatography with 11 fractions. In that E:M, 16:4, 12:8, 10:10, which showed maximum zone of inhibition against E. coli (4 mm), K. oxytoca (4 mm), K. pneumoniae (3 mm), L. vulgaris (4 mm) and V. parahemolyticus (5 mm). 18:2, 8:12, 6:14, 4:12 and 2:18 with these fractions showed minimum activities against the all pathogenic microbial forms.
Keywords:
Antibacterial activities, gastropod, Hemifusus pugilinus, pathogens,
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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