Research Article | OPEN ACCESS
Phytochemical Analysis and in vitro Antifungal Profile of Bioactive Fractions from Sterculia setigera (Sterculiaceae)
1M. Ouedraogo, 2K. Konate, 3P. Zerbo, 4N. Barro and 1Laya L. Sawadogo
1Laboratory of Animal Physiology
2Laboratory of Biochemistry and Applied Chemistry
3Laboratory of Plants Ecology and Biology, University of Ouagadougou,
09 P.O. Box: 848, Ouagadougou 09, Burkina Faso
4Laboratory of Biochemistry and Molecular Genetics Microbial, University of
Ouagadougou, 03 P.O. Box: 7131, Ouagadougou 03, Burkina Faso
Current Research Journal of Biological Sciences 2013 2:75-80
Received: November 19, 2012 | Accepted: January 01, 2013 | Published: March 20, 2013
Abstract
Sterculia setigera L. is a small evergreen tree having antimicrobial, anti-inflammatory effects. Leaves of Sterculia setigera have been investigated for its phytochemical and antifungal properties. In the present study, aqueous acetone extract and fractions of leaf from Sterculia setigera were used. Quantitative analysis of extract and fractions revealed the presence of total phenolic and total flavonoid. The polyphenol content was estimated by Folin-ciocalteu and AlCl3 methods aim of this present study was access the phytochemical composition and antifungal properties of extract and fractions from this Sterculiaceae. Folin-ciocalteu and AlCl3 methods respectively were used for polyphenol contents. The antifungal potency was investigated by Micro-well dilution (MIC) and Minimum Fungicidal Concentration (MFC) assay against eight different fungal species. Results indicated that total phenolic and flavonoid contents revealed that EAF and DCMF have the highest phenolic and flavonoid contents 30.02±1.02 mg GAE and 8.24±1.10 mg QE, respectively. As for the Minimum Inhibitory Concentration assay (MIC) and Minimum Fungicidal Concentration (MFC) of extract and fractions, result varied according to microorganism. The MIC values of fractions were ranged from 31.25 to 500 μg/mL and as for the MFC values were ranged from 125 to 1000 μg/mL. Among the samples tested for antifungal activity, EAE and DCMF have also the highest activities compared to other fractions. In short, our findings revealed that DCMF and EAF have influenced the antifungal properties. As a matter of fact, Sterculia setigera could be exploited to formulate novel drugs from plan origin against fungal infections.
Keywords:
Antifungal activity, polyphenol content, Sterculia setigera L,
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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