Abstract

This study was carried out to investigate, the protective potential of Allium sativum on the stomach following ethanol-induced mucosal lesions in Adult male wistar rats (Rattus novergicus). Twenty four Adult male Wistar rats were used in the study and divided randomly into 3 groups (8 animals in each); Group A received orally, Phosphate Buffered Saline (PBS) and served as control; groups B and C received 1.0 mL of 35% ethanol orally every 24 h and group C received freshly prepared Garlic Homogenate (FGH) (300 mg/kg/body weight) once daily one hour prior to ethanol administration. The study was for 21 days after which the animals were sacrificed following cervical dislocation and the stomach being the organ of study were excised. The excised stomach was homogenised in 5% sucrose solution for biochemical studies and there was significant increase in the level of GSH-Px of FGH treated group when compared to those treated with ethanol only. Marked increase in the level of G-6-PDH and TBARS in ethanol treated group was observed compared to FGH treated and the control groups.

Keywords:

Allium sativum, glucose-6-phosphate dehydrogenase (G-6-PDH), gluthathion peroxidise (GSH-Px), Rattus novergicus, Stomach, thiobabituric acid (TBARS),

References

1. Borek, C., 2001. Antioxidant health effects of aged garlic extract. J. Nutr., 131(3Suppl): 1010S-1015S.
   CrossRef    PMid:11238807    
2. Cavallito, C., J.H. Bailey and J.S. Buck, 1944. Allicin, the antibacterial principle of Allium sativum II. Its precursor and ''essential oil'' of garlic. J. Am. Chem. Soc., 67: 1032-1033.
   CrossRef    
3. Gaffen, J.D., I.A. Tavares and A. Bennett, 1984. The effect of garlic extract on contractions of rat gastric fundus and human platelet aggregation. J. Pharm. Pharmacol., 36(4): 272-274.
   CrossRef    PMid:6144781    
4. Halliwell, B., J.M.C. Gutteridge and C.E. Cross, 1992. Free radicals, antioxidants and human disease: Where are we now? J. Lab. Clin. Med., 119(6): 598-620.
   PMid:1593209    
5. Harris, J.C., S.L. Cotrell, S. Plummer and D. Lloyd, 2001. Antimicrobial properties of Allium sativum (garlic). Appl. Microbiol. Biotechnol., 57(3): 382-386.
   CrossRef    
6. Hoshino, T., N. Kashimoto and S. Kasuga, 2001. Effects of garlic preparations on the gastrointestinal mucosa. J. Nutr., 131: 1109S-13S.
   CrossRef    PMid:11238827    
7. Kosuge, Y., Y. Koen, K. Ishige, K. Minami, H. Urasawa, H. Saito and Y. Ito, 2003 S-Allyl-L-cysteine selectively protects cultured rat hippocampal neurons from amyloid beta-protein- and tunicamycininduced neuronal death. Neuroscience, 122: 885-895.
   CrossRef    PMid:14643758    
8. Kraft, K., 2009. Complementary/alternative medicine in the context of prevention of diseases and maintenance of health. Prev. Med., 49(2-3): 88-92.
   CrossRef    PMid:19465045    
9. Moriguchi, T., K. Takashina, P.J. Chu, H. Saito and N. Nishiyama, 1994. Prolongation of life span and improved learning in the senescence accelerated mouse produced by aged garlic extract. Biol. Pharm. Bull., 17: 1589-1594.
   CrossRef    PMid:7735200    
10. Rodwell, V., 1993. Emzymes; General Properties. Harpers Biochemistry. 24th (Edn.), Prentice-Hall Inc., pp: 64.
    
11. Ross, I.A., 1999. Medicinal plants of the world. Humana Press, New Jersey, USA, pp: 25-63.
    
12. Sheela, C.G., K. Kumud and K.T. Augusti, 1995. Antidiabetic effect of onion and garlic sulfoxide aminoacid in rats. J. Planta. Med., 61: 356-357.
    CrossRef    PMid:7480182    
13. Silagy, C. and A. Niel, 1994. A Meta analysis of the effect of garlic on blood pressure. J. Hypertens., 12: 463-468.
    CrossRef    PMid:8064171    

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