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     International Journal of Animal and Veterinary Advances


Identification of Novel Milk Protein Gene Variants in Sahiwal Cattle Breed of Pakistan

1Shahlla N. Mir, 2Mohammad A. Tahir and 1Riazuddin Sheikh
1Center of Excellence in Molecular Biology, University of the Punjab, 87 West Canal Bank Road, Lahore, Pakistan
2Punjab Forensic Science Agency, Thokar Niaz Baig, Multan Road, Lahore, Pakistan
International Journal of Animal and Veterinary Advances  2013  1:38-43
http://dx.doi.org/10.19026/ijava.5.5575  |  © The Author(s) 2013
Received: January 10, 2013  |  Accepted: February 08, 2013  |  Published: February 20, 2013

Abstract

This novel study was aimed at identification of new genetic variants in Sahiwal cattle breed of Pakistan and determined the effects of these variants on milk yield. Five major milk protein genes in Sahiwal cattle were analyzed and two single nucleotide polymorphisms identified through bi-directional sequencing. These include A to T in exon XI at position 11462 of the alpha s1 casein gene; resulting in a Glutamic Acid (GAA) to Aspartic acid (GAU) substitution at position 84 of alpha s1 casein protein and T to C change at position 8491 of the exon VII in beta-casein gene resulting in a Valine to Alanine substitution at position 197 of beta casein protein. Amplification Refractory Mutation System (ARMS) and SNaPshot genotyping protocols were optimized for genotyping new genetic variants. The genotypes in both the alpha-s1 casein and beta casein genes were found associated with milk yield but their influence was not statistically significant. However, the least square means of milk yield for TT genotypes of alpha s1 casein and of beta casein genes were higher compared to other genotypes.

Keywords:

&alphas1-casein, &beta-casein, genetic variant, sahiwal cattle,


References


Competing interests

The authors have no competing interests.

Open Access Policy

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Copyright

The authors have no competing interests.

ISSN (Online):  2041-2908
ISSN (Print):   2041-2894
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