Research Article | OPEN ACCESS
Effects of Inclusion of Two Probiotic Strains Isolated From “Sha’aâ€, a Maize-Based
Traditionally Fermented Beverage on Lipid Metabolism of Rabbits fed a Cholesterol-Enriched Diet
1Zambou Ngoufack Francois, 1Kaktcham Pierre Marie, 2Fonteh Anyangwe Florence, 1Guetiya Wadoum Raoul and 1Sieladie Djomne Victor
1Laboratory of Biochemistry, Food Science and Nutrition (LABPMAN), Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67 Dschang, Cameroon
2Department of animal Production, Faculty of Agronomy and Agricultural Sciences, University of Dschang, P.O. Box 96 Dschang, Cameroon
International Journal of Animal and Veterinary Advances 2013 2:87-97
Received: February 06, 2013 | Accepted: March 08, 2013 | Published: April 20, 2013
Abstract
The objective of this study was to assess the in vitro probiotic potential of Lactobacillus plantarum Lp10S and Lp11S isolated from Sha’a and evaluate their effects on lipid metabolism of hypercholesterolaemic rabbits. The strains were tested in vitro for their acid and bile tolerance, capability to remove cholesterol from MRS broth and to deconjugate bile salts. For in vivo studies, Lactobacillus plantarum Lp10S and Lp11S were administered to rabbits fed on cholesterol-enriched diet (standard diet+2×0.02 g of pure cholesterol per day per rabbit) at a dose of 2×108 CFU per day per rabbit for each strain; the treatment lasted seven weeks and one week post ingestion period was observed. Serum lipids were analyzed during the experiment. The results revealed that strains Lactobacillus plantarum Lp10S and Lp11S tolerated well low pH and bile salts, expressed bile salt hydrolase activity and have ability to assimilate cholesterol in vitro. The results also showed that diets including Lactobacillus plantarum Lp10S and Lp11S significantly lowered serum total cholesterol, (VLDL+IDL+LDL)-cholesterol and triglycerides levels of hypercholesterolaemic rabbits. In addition, HDL-cholesterol level significantly increased and the atherosclerosis index was significantly lowered. The present results indicate the probiotic potential of Lactobacillus plantarum Lp10S and Lp11S strains as well as their in vivo cholesterol-lowering properties that may be useful for their application as health-promoting bacteria.
Keywords:
Cholesterol-lowering effect, Lactobacillus plantarum, Probiotics, rabbits, Sha,
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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ISSN (Online): 2041-2908
ISSN (Print): 2041-2894 |
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