Research Article | OPEN ACCESS
Clinico-pathological Changes Associated with Brucella melitensis Infection and its Bacterial Lipopolysaccharides (LPS) in Male Mice
1,3Faez Firdaus Jesse Abdullah, 1Norasiah Binti Nik, 3Mohd Zamri Saad, 1Abd Wahid Haron, 2,4Abdul Rahman Omar, 1Jasni Sabri, 1Lawan Adamu, 1Abdinasir Yusuf Osman and 1Abdul Aziz Saharee
1Department of Veterinary Clinical Studies,
2Department of Veterinary Pathology and Microbiology, Faculty of Veterinary
Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
3Research Centre for Ruminant Disease UPM, Selangor, Malaysia
4Institute of Bioscience UPM, Selangor, Malaysia
International Journal of Animal and Veterinary Advances 2013 5:165-170
Received: February 27, 2013 | Accepted: March 27, 2013 | Published: October 20, 2013
Abstract
Brucella melitensis (B. melitensis) is gram negative, aerobic bacteria that cause Brucellosis in humans’ sheep and goats. Brucellosis causes abortion in wild and domestic animals resulting in enormous financial losses. Therefore, the purpose of this study was to evaluate the clinico-pathological changes associated with Brucella melitensis infection and its bacterial Lipopolysaccharides (LPS) in male mice. Three groups of 24 Balb/c male mice consisting of 8 mice in each group were used as an animal model for the study. The control group were inoculated intraperitoneally with 1 mL of Phosphate Buffered Solution (PBS) pH 7 while, the treatment groups were inoculated intraperitoneally with 1 mL×109 of B. melitensis colony and 1 mL×109 of Lipopolysaccharides (LPS) extracted from B. melitensis respectively. Mice that showed severe clinical signs and those that survived were euthanized by cervical dislocation method after 5 days of post infection subsequently, post mortem was conducted and histopathological studies were carried out. B. melitensis group showed severe clinical signs between 6 to 17 h of post inoculation compared to the PBS and LPS groups. The LPS group became lethargic 2 h post inoculation but, they become active after 5 h post inoculation, while the control group (PBS) exhibited normal responses. Histopathology results showed severe tissue alterations in the reproductive organs of the B. melitensis group compared to LPS group. In conclusion, the atrophy of the spermatocytes in the testes and degenerative necrosis of the pseudo stratified epithelium of the vas deferens in the B. melitensis group were severe while, LPS group showed moderate atrophy of the spermatocyte of the testes and severe degenerative necrosis of the pseudo stratified epithelium of the vas deferens.
Keywords:
Atrophy, B. melitensis, brucellosis, lipopolysaccharides, spermatocyte, vas deferens,
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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ISSN (Online): 2041-2908
ISSN (Print): 2041-2894 |
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