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     International Journal of Animal and Veterinary Advances


Gene Therapy Trail for Treatment of Tumor in Pregnant and Non Pregnant Mice by Mixed DNA with RNA

Saad. A. Hatif
College of Vet Med, Baghdad University, Corniche St, Baghdad, Iraq
International Journal of Animal and Veterinary Advances  2013  5:199-201
http://dx.doi.org/10.19026/ijava.5.5597  |  © The Author(s) 2013
Received: May 10, 2013  |  Accepted: June 06, 2013  |  Published: October 20, 2013

Abstract

This study was conducted on 35 Adult Mus Musculus (Swiss albino) mice, it was suffering from tumors (parotid gland adenocarcinoma and another subcutaneous tumor). The mice divided in two groups, 25 mice treated and 10 mice control group. Total genome extracted from liver of camel by lysis buffer solution (100 mM NaCl, 1 mM EDTA, 10 mM Tris-HCl pH 8.0) 5% SDS and purification by chloroform-phenol. Treated group subjected for gene therapy by in situ injection of 0.1cc mixed camel nucleic acid three times, until tumor mass disappear. All mice for control group were dead, while the mice treated still alive. In conclusion, the total genome of camel may play an important role in the defense mechanism of the body against adinoarcinoma.

Keywords:

Gene therapy, pregnant mice, tumor,


References


Competing interests

The authors have no competing interests.

Open Access Policy

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Copyright

The authors have no competing interests.

ISSN (Online):  2041-2908
ISSN (Print):   2041-2894
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