Research Article | OPEN ACCESS
Evaluation of the Antimicrobial Activity of the K9CATH Peptide (38 Amino Acids) Against a Mastitis Isolated Strain of Staphylococcus aureus by the Resazurin microtiter Method
1Albero Barreras-Serrano, 1Alma Rossana Tamayo-Sosa, 1Victor Manuel del Villar-Perez, 1Alma Angelina Castellanos-Felix, 1Luis Tinoco-Gracia and 2Tonatiuh Melgarejo
1Research Institute of Veterinary Science, University of Baja California, Mexicali, BC Mexico
2Department of Human Nutrition, Kansas State University, Manhattan, KS, USA
International Journal of Animal and Veterinary Advances 2014 2:58-60
Received: December 28, 2012 | Accepted: January 17, 2013 | Published: April 20, 2014
Abstract
The antimicrobial activity of the synthetic peptide K9CATH was determined by the Resazurin microtitre Method (RMM) against a strain of S. aureus isolated from a case of mastitis. To the antibiogram this bacteria strain showed to be resistant to Ampicillin, Erythromycin, Cefeprime, Dicloxaciline and Penicillin (10 U), while the MIC obtained for the K9CATH was 5.66 &mug/mL. Unlike the reference broth method, visual reading for MIC determination with the RMM showed to be easier, rapid, inexpensive and more sensitive for antimicrobial peptide screening, based in a color change from blue (not growth) to pink (growth). This is the first time that the resazurin method is used to determine the MIC of the 38 aas K9CATH peptide against a mastitic isolate of S. aureus.
Keywords:
AMPs and mastitis, K9CATH peptide, resazurin assay, Staphylococcus aureus,
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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ISSN (Online): 2041-2908
ISSN (Print): 2041-2894 |
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