Research Article | OPEN ACCESS
Optimization of Cellulase Production by Trichoderma reesei HY07 Using Response Surface Methodology
Guowei Shu, Hui Yang, He Chen and Shikai Wang
College of Life Science and Engineering, Shaanxi University of Science and Technology,
Xi’an 710021, China
Research Journal of Applied Sciences, Engineering and Technology 2013 23:5438-5442
Received: November 24, 2012 | Accepted: January 17, 2013 | Published: May 28, 2013
Abstract
Response Surface Methodology (RSM) based on a three-level, three-variable Box and Behnken Factorial Design (BBFD) was used to evaluate the interactive effects of corn stalk: bran ratio, tween80 and temperature on the cellulase production by solid fermentation. The optimum conditions derived via RSM were: corn stalk: bran ratio 1.29:1, Tween80 11.05 &muL and temperature 31°C for carboxymethyl cellulase (CMCase) and corn stalk: bran ratio 0.77:1, Tween80 12.54μL and temperature 32°C for Filter Paper Activity (FPA). The actual experimental yield was 406.42 U/g for CMCase and 93.62 U/g for FPA under optimum condition, which compared well to the maximum predicted value of 405.67 U/g and 91.29 U/g. The cellulase yield under optimal conditions was 1.45 fold for CMCase and 1.33 fold for FPA to the control.
Keywords:
Cellulose, carboxymethyl cellulose, FPA, response surface methodology, solid state fermentation,
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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ISSN (Online): 2040-7467
ISSN (Print): 2040-7459 |
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