Abstract

GSH is one of the most ubiquitous proteins known to provide protection against toxic heavy metals. A gene-AtGCS encoding Arabidopsis &gamma-glutamylcysteine synthetase was introduced into Escherichia coli (BL21) by over-expression of TrxA-AtGCS fusion protein and analyzed the tolerant capacity to heavy metal by transformed E. coli. As comparison, the strain over-expressing TrxA was selected as the control. The results showed that the growth of E. coli cells over-expressing TrxA-AtGCS was much better than the control cells expressing TrxA under 1 mM Cd²⁺, Zn²⁺ and Cu²⁺ heavy metals respectively. Meanwhile, the higher bioaccumulation of Cd²⁺, Zn²⁺, Cu²⁺ and GSH content were observed in the strain over-expressing TrxA-AtGCS. It could be concluded that over-expression of AtGCS offered a promising heavy metals resistance of E. coli with superior heavy metals accumulation and GSH content.

Keywords:

Accumulation, AtGCS, E. coli, heavy metals tolerance, GSH content,

References

Competing interests

The authors have no competing interests.

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