Abstract

Cellulose can be degraded by enzyme to glucose, which provide carbon source for ethanol fermentation. This study, taking Burmuda grass as material, analyzed effects of temperature, time, pH, PBS dosage and ratio of enzyme on reducing sugar. It came to the conclusion that the best conditions of the enzymolysis of Burmuda grass are as follows: 50°C for temperature, 32 h for time, 4.20 for pH, 2 mL for PBS dosage, 4:3 for the ratio of xylanase and cellulase, the yield of reducing sugar reached 78.63%.

Keywords:

Bioethanol, cellulose, orthogonal experiment, reducing sugar, standard curve, xylanase and cellulase,

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Competing interests

The authors have no competing interests.

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