Research Article | OPEN ACCESS
Bioinformatics Analysis of the Duck Enteritis Virus UL54 Gene
1, 3Chaoyue Liu, 1, 2, 3Anchun Cheng and 1, 2, 3Mingshu Wang
1Institute of Preventive Veterinary Medicine
2Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, P.R. China
3Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, 46 Xinkang Road, Ya
Research Journal of Applied Sciences, Engineering and Technology 2014 14:2813-2817
Received: October 31, 2012 | Accepted: January 03, 2013 | Published: April 12, 2014
Abstract
In this study, we analyze the Duck Enteritis Virus (DEV) UL54 gene, which has been isolated and identified in our lab (GenBank accession NO EU071033), to help deeply research on DEV. DNA sequence analysis showed that the identified ORF which composed of 1377 bp nucleotides encoded 458 amino acids with a predicted Mr. of 51.75 kDa. Multiple sequence alignment suggested that the UL54 gene was highly conserved in Alphaherpesvirinae and was similar to the other herpesviral UL54 gene. Phylogenetic analysis of the DEV UL54 gene revealed that DEV had a close evolutionary relationship with Gallid, Herpesvirus 2 (GaHV-2), Gallid Herpesvirus 3 (GaHV-3), Meleagrid Herpesvirus1 (MeHV-1) and should belong to a single cluster within the Alphaherpesvirinae subfamily.
Keywords:
DEV, molecular characterization, sequence analysis, UL54,
References
-
Anchun, C., W. Mingshu and W. Ming, 2006. Construction of duck enteritis virus gene libraries and discovery: Cloning and identification of viral nucleocapsid protein gene. High Technol. Lett., 16: 948-953.
-
Andrew, J. and Davison, 2010. Herpesvirus systematic. Vet. Microbiol., 143: 52-69.
CrossRef PMid:20346601 PMCid:PMC2995426
-
Bei, L., X. Chao and C. Anchun, 2010. Identification and characterization of duck plague virus glycoprotein C gene and gene product. Virol. J., 7: 349.
CrossRef PMid:21110887 PMCid:PMC3004831
-
Chanjuan, S., C. Anchun and W. Mingshu, 2009. Identification and characterization of the duck, enteritis virus UL51 gene. Arch Virol., 154: 1061-1069.
CrossRef PMid:19517212
-
Corbin-Lickfett, K.A., S.K. Souki and M.J. Cocco, 2010. Three Arginine residues within the RGG Box is crucial for ICP27 binding to Herpes Simplex Virus 1 GC-rich sequences and for efficient viral RNA export. Virol. J., 84: 6367-6376.
CrossRef PMid:20410270 PMCid:PMC2903288
-
Corbin-Lickfett, K.A., I.H. Chen, M.J. Cocco and R.M. Sandri-Goldin, 2009. The HSV-1 ICP27 RGG box specifically binds flexible, GC-rich sequences but not G-quartet structures. Nucl. Acids Res., 37(21): 7290-7301.
CrossRef PMid:19783816 PMCid:PMC2790906
-
Hernandez, F.P. and R.M. Sandri-Goldin, 2010. Herpes Simplex Virus 1 regulatory protein ICP27 undergoes a head-to-tail intramolecular interaction. Virol. J., 84: 4124-4135.
CrossRef PMid:20164236 PMCid:PMC2863791
-
Hua, C., C. Anchun and W. Mingshu, 2009. Complete nucleotide sequence of the duck plague, virus gE gene. Arch Virol., 154: 163-165.
CrossRef PMid:19089586
-
Hua, C., C. Anchun and W. Mingshu, 2010. Cloning, expression and characterization of gE protein of Duck plague virus. Virol. J., 7: 120.
CrossRef PMid:20529349 PMCid:PMC2897786
-
Judith, B., G.K. Barbara and C.M. Thomas, 1995. Pseudorabies virus and equine herpesvirus 1 share a nonessential gene which is absent in other herpesviruses and located adjacent to a highly conserved gene cluster. Virol. J., 69: 5560-5567.
-
King, A., E. Lefkowitz and M.J. Adams, 2011. Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, San Diego.
-
Lichan, Z., C. Anchun and W. Mingshu, 2008a. Characterization of codon usage bias in the dUTPase gene of duck enteritis virus. Prog. Nat. Sci., 18: 1069-1076.
CrossRef
-
Lichan, Z., C. Anchun and W. Mingshu, 2008b. Identification and characterizeation of duck enteritis virus dUTPase gene. Avian Dis., 52: 324-331.
CrossRef PMid:18646465
-
Liting, L., C. Anchun and W. Mingshu, 2010. RPoly clonal antibody against the DPV UL46M: Protein can be a diagnostic candidate. Virol. J., 7: 83.
CrossRef PMid:20429885 PMCid:PMC2867803
-
Meili, L., W. Shuai and C. Mingsheng, 2011. Identification of nuclear and nucleolar localization signals of Pseudo Rabies Virus (PRV) early protein UL54 reveals that its nuclear targeting is required for efficient production of PRV. Virol. J., 85: 10239-10251.
CrossRef PMid:21795331 PMCid:PMC3196411
-
Mingsheng, C., C. Anchun and W. Mingshu, 2009. Characterization of synonymous codon usage bias in the duck plague virus UL35Gene. Intervirology, 52: 266-278.
CrossRef PMid:19672100
-
Renyong, J., C. Anchun and W. Mingshu, 2009. Analysis of synonymous codon usage in the UL24 gene of duck enteritis virus. Virus Gen., 38: 96-103.
CrossRef PMid:18958612
-
Sandhu, T.S. and L. Leibovitz, 2008. Duck virus enteritis (Duck Plauge). Dis. Poultry, pp: 384-393.
-
Shunchuan, Z., M. Guangpeng and X. Jun, 2010. Expressing gK gene of duck enteritis virus guided by bioinformatics and its applied prospect in diagnosis. Virol. J., 7: 168.
CrossRef PMid:20663161 PMCid:PMC2921365
-
Wei, X., C. Anchun and W. Mingshu, 2010. Molecular cloning and characterization of the UL31 gene rom Duck enteritis virus. Mol. Biol. Rep., 37: 1495-1503.
CrossRef PMid:19466580
-
Yaju, H., C. Mawsheng and W. Chingying, 2005. Mapping of functional regions conferring: Nuclear localization and RNA-binding activity of pseudorabies virus early protein UL54. J. Virol. Methods, 130: 102-107.
CrossRef PMid:16054707
-
Ying, W., C. Anchun and W. Mingshu, 2011. Characterization of the duck enteritis virus UL55 protein. Virol. J., 8: 256.
CrossRef PMid:21609474 PMCid:PMC3117846
-
Ying, W., C. Anchun and W. Mingshu, 2012. Complete genomic sequence of Chinese virulent: Duck enteritis virus CHv strain. Virol. J., 10: 5965.
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
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The authors have no competing interests.
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