Research Article | OPEN ACCESS
Enhancement of Rice Seed Germination and Vigour by Trichoderma spp.
1Febri Doni, 2I. Anizan, 1C.M.Z. Che Radziah, 1Ahmad Hilmi Salman, 2Muhammad Hidayat Rodzihan and 1Wan Mohtar Wan Yusoff
1School of Biosciences and Biotechnology
2Department of Geology, School of Environmental and Natural Resources Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
Research Journal of Applied Sciences, Engineering and Technology 2014 21:4547-4552
Received: January 04, 2014 | Accepted: January 17, 2014 | Published: June 05, 2014
Abstract
The present study was undertaken to examine the effectiveness of Trichoderma spp. to enhance rice germination and vigour. An in vitro experiment was carried out to assess the effect of seven isolates of Trichoderma spp. in enhancing rice germination and vigour. The results showed that all isolates of Trichoderma spp. significantly increased rice seedling growth, germination rate, vigour index and speed of germination with Trichoderma sp., SL2 showing the greatest increase in all the four parameters. Trichoderma sp., SL2 treated rice seeds attained values of 4.48 and 6.00 cm, 0.0084 and 0.0048 g and 1016.56 and 44.75 seeds/day for seedling shoot length seedling root length, shoot weight, root weight, vigour index and speed of germination respectively. We may conclude that Trichodermaa spp. is able to enhance seed germination and vigour. The results of the study adds to the further understanding of the role of beneficial fungi in improving rice resistance to stress, yield and quality through seed invigoration.
Keywords:
Germination, rice, Trichoderma spp, vigour,
References
-
Abul-Baki, A.A. and J.D. Anderson, 1973. Vigour determination in soybean by multiple criteria. Crop Sci., 3: 630-637.
CrossRef
-
Adesemoye, A.O. and J.W. Kloepper, 2009. Plant-microbes interactions in enhanced fertilizer-use efficiency. Appl. Microbiol. Biot., 85: 1-12.
CrossRef PMid:19707753
-
Anwar, S., M. Iqbal, S.H. Raza and N. Iqbal, 2013. Efficacy of seed preconditioning with salicylic and ascorbic acid in increasing vigor of rice (Oryza sativa L.) seedling. Pak. J. Bot., 45(1): 157-162.
-
Asaduzzaman, M., M.J. Alam and M.M. Islam, 2010. Effect of Trichoderma on seed germination and seedling parameters of chili. J. Sci. Found., 8(1/2): 141-150.
-
Basra, S.M.A., M. Ashraf, N. Iqbal, A. Khaliq and R. Ahmad, 2004. Physiological and biochemical aspects of pre-sowing heat strees on cotton seed. Seed Sci. Technol., 32: 365-774.
CrossRef
-
Bomke, C. and B. Tudzynski, 2009. Diversity, regulation and evolution of the gibberellin biosynthetic pathway in fungi compared to plants and bacteria. Phytochemistry, 70: 1876-1893.
CrossRef PMid:19560174
-
Bradford, K.J., 1986. Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. HortScience, 21: 1105-1112.
-
Cai, F., G. Yu, P. Wang, Z. Wei, L. Fu, Q. Shen and W. Chen, 2013. Harzianolide, a novel plant growth regulator and systemic resistance elicitor from Trichoderma harzianum. Plant Physiol. Bioch., 73: 106-113.
CrossRef PMid:24080397
-
Chowdappa, P., S.P.M. Kumar, M.J. Lakshmi and K.K. Upreti, 2013. Growth stimulation and induction of systemic resistance in tomato against early and late blight by Bacillus subtilis OTPB1 or Trichoderma harzianum OTPB3. Biol. Control, 65: 109-117.
CrossRef
-
Dash, A.K., 2012. Impact of domestic waste water on seed germination and physiological parameters of rice and wheat. Int. J. Res. Rev. Appl. Sci., 12(2): 280-286.
-
Doni, F., N.K.N. Al-Shorgani, E.M.M. Tibin, N.N. Abuelhassan, I. Anizan, C.M.Z. Che Radziah and W.Y. Wan Mohtar, 2013. Microbial involvement in growth of paddy. Curr. Res. J. Biol. Sci., 5(6): 285-290.
-
FAO, 2012. FAO Says Rice Production Outpacing Consumption.
Direct Link
-
Farooq, M., S.M.A. Basra, A. Wahid, A. Khaliq and N. Kobayashi, 2010. Seed Invigoration: A Review. In: Lichtfouse, E. (Ed.), Organic Farming, Pest Control and Remediation of Soil. Springer, NY, pp: 137-175.
-
Goswami, A., R. Banerjee and S. Raha, 2013. Drought resistance in rice seedlings conferred by seed priming. Protoplasma, 250(5): 1115-1119.
CrossRef PMid:23443969
-
Gupta, P.C., 1993. Seed Vigour Testing. In: Agarwal, P.K. (Ed.), Handbook of Seed Testing. National Seed Corporation, New Delhi, pp: 245-246.
PMid:8517121
-
Harman, G.E., 2000. Myths and dogmas of biocontrol changes in perception derived from research on Trichoderma harzianum T-22. Plant Dis., 84: 377-393.
CrossRef
-
Harman, G.E., R. Petzoldt, A. Comis and J. Chen. 2004. Interactions between Trichoderma harzianum strain T22 and maized inbred line Mo17 and effect of this interaction on diseases caused by Pythium ultimum and Colletotrichum graminicola. Phytopathology, 94: 147-153.
CrossRef PMid:18943537
-
IRRI, 2011. Measuring Seed Germination. Post Harvest Fact Sheets.
Direct Link
-
Jayalakshmi, S.K., S. Raju, R. Usha, V.I. Benagi and K. Sreeramulu, 2009. Trichoderma harzianum L1 as a potential source for lytic enzymes and elicitor of defense responses in chickpea (Cicer arietinum L.) against wilt disease caused by Fusarium oxysporum f. sp. ciceri. Aust. J. Crop Sci., 3(1): 44-52.
-
Jiang, X., A. Geng, N. He and Q. Li, 2011. New isolates Trichoderma viride strain for enhanced cellulolytic enzyme complex production. J. Biosci. Bioeng., 111(2): 121-127.
CrossRef PMid:21071269
-
Juraimi, A.S., M.P. Anwar, A. Selamat, A. Puteh and A. Man. 2012. The influence of seed priming on weed supression in aerobic rice. Pak. J. Weed Sci. Res., 18: 257-264.
-
Lalitha, P., Srujana and K. Arunalakshmi, 2012. Effect of Trichoderma viride on germination of mustard and survival of mustard seedlings. Int. J. Life Sci. Biotechnol. Pharma Res., 1(1).
-
Martínez-Medina, A., A. Roldán, A. Albacete and J.A. Pascual, 2011. The interaction with arbuscular mycorrhizal fungi or Trichoderma harzianum alters the shoot hormonal profile in melon plants. Phytochemistry, 72: 223-229.
CrossRef PMid:21145086
-
Mukherjee, P.K., C.S. Nautiyal and A.N. Mukhopadhyay, 2008. Molecular Mechanisms of Biocontrol by Trichoderma spp. In: Nautiyal, C.S. (Ed.), Molecular Mechanisms of Plant and Microbe Coexistence. Soil Biology 15, Springer-Verlag, Berlin, Heidelberg, DOI: 10.1007/978-3-540-75575-3.
CrossRef
-
Okoth, S.A., J.A. Otadoh and J.O. Ochanda, 2011. Improved seedling emergence and growth of maize and beans by Trichoderma harziunum. Trop. Subtrop. Agroecosyst., 13: 65-71.
-
Phinney, B.O., 1983. The History of Gibberellins. In: Crozier, A. (Ed.), The Biochemistry and Physiology of Gibberellins. Praeger Publishers, New York, pp: 19-52.
-
Rajjou, L., M. Duval, K. Gallardo, J. Catusse, J. Bally, C. Job and D. Job, 2012. Seed germination and vigor. Ann. Rev. Plant Biol., 63: 507-533.
CrossRef PMid:22136565
-
Saba, H., D. Vibhash, M. Manisha, K.S. Prashant, H. Farham and A. Tauseff, 2012. Trichoderma-a promising plant growth stimulator and biocontrol agent. Mycosphere, DOI: 10.5943/mycosphere/ 3/4/14.
CrossRef
-
San-Oh, Y., T. Sugiyama, D. Yoshita, T. Ookawa and T. Hirasawa, 2006. The effect of planting pattern on the rate of photosynthesis and related processes during ripening in rice plants. Field Crop Res., 96: 113-124.
CrossRef
-
Saravanakumar, K., V. Shanmuga Arasu and K. Kathiresan, 2013. Effect of trichoderma on soil phosphate solubilization and growth improvement of Avicennia marina. Aquat. Bot., 104: 101-105.
CrossRef
-
Tančić, S., J. Skrobonja, M. Lalošević, R. Jevtić and M. Vidić, 2013. Impact of Trichoderma spp. on soybean seed germination and potential antagonistic effect on Sclerotinia sclerotiorum. Pestic. Phytomed., 28(3): 181-185.
CrossRef
-
Yadidia, I., N. Benhamou and I. Chet, 1999. Induction of defense responses in cucumbar plants (Cucumis sativus L.) by biocontrol agent Trichoderma harzianum. Appl. Environ. Microb., 65: 1061-1070.
-
Zarei, M. and M. Sinaki, 2012. Effect of priming on seed germination rice (Oryza sativa L.) in difficult environment conditions. Int. J. Agric. Res. Rev., 2(S): 1070-1078.
-
Zheng, Z. and K. Shetty, 2000. Enhancement of pea (Pisum sativum) seedling vigour and associated phenolic content by extracts of apple pomace fermented with Trichoderma spp. Process Biochem., 36: 79-84.
CrossRef
Competing interests
The authors have no competing interests.
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