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     Research Journal of Environmental and Earth Sciences


Growth Dynamics of Bacteria Isolated from Spent Engine Oil Contaminated Tropical Soil

1C. Nwinyi Obinna, 1Ajaja Olaleye and 2Nwinyi Chibuzo
1Department of Biological Sciences, School of Natural and Applied Sciences, College of Science and Technology, Covenant University, KM 10 Idiroko Road, Canaan Land, PMB 1023 Ota, Ogun State
2 Nigerian Security and Civil Defence Corps, Anambra State, Nigeria
Research Journal of Environmental and Earth Sciences  2014  8:430-436
http://dx.doi.org/10.19026/rjees.6.5254  |  © The Author(s) 2014
Received: April ‎29, ‎2014  |  Accepted: May ‎25, ‎2014  |  Published: September 20, 2014

Abstract

Sites contaminated with spent oil in Canaan land Ota Nigeria were screened for the presence of spent oil degrading bacteria. The method of continual enrichment on the spent oil yielded five pure cultures that were selected for further physiological studies. From the morphological and biochemical characterization and comparison with respect to the standard references, the isolates were most likely the members of the genera Acinetobacter, Arthrobacter, Bacillus, Mycobacterium and Pseudomonas species. Time course studies of these organisms on the spent oil monitored by OD and pH fluxes resulted in exponential increases in the turbidity and steady declines in the pH. The mean OD obtained was 0.147±0.052-1.591±0.320 with pH 7.31±0.01-6.30±0.03. From the study, it was evident that bacterial species in tropical ecosystem can be explored for the recovery of sites polluted with spent engine oil.

Keywords:

Biostimulation, enrichment, ecosystem, ecosystem, optical density, spent engine oil,


References


Competing interests

The authors have no competing interests.

Open Access Policy

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Copyright

The authors have no competing interests.

ISSN (Online):  2041-0492
ISSN (Print):   2041-0484
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