Research Article | OPEN ACCESS
Amelioration of High Cholesterol Diet Caused Lipids Accumulation in Hepatic Cells by Rutin and Ascorbic Acid
Abdulaziz M. Aleisa
Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University,
P.O. Box 2457, Riyadh 11451, Saudi Arabia, Tel.: +96614677178, Fax: +96614677200
British Journal of Pharmacology and Toxicology 2013 2:56-64
Received: November 24, 2012 | Accepted: January 11, 2013 | Published: April 25, 2013
Abstract
Non Alcoholic Fatty Liver Disease (NAFLD) has become a very common metabolic disorder. It refers to a group of conditions where excess fats are deposited in hepatic cells. Several approaches have been considered for the management of NAFLD including dietary changes, which were reported to suppress hepatic lipids accumulation in previous studies. The present study was designed to investigate the possible synergistic effects of Rutin (RT) and Ascorbic Acid (AA) against lipids accumulation in hepatic cells of male Wistar albino rats. Thirty animals received freshly prepared experimental High Cholesterol Diets (HCD) with or without RT and/or AA for 6 consecutive weeks. In hepatic tissues nucleic acids, total protein, Total Cholesterol (TC) and Triglycerides (TG) concentrations were measured. Histopathological changes were also observed. In hepatic cells, nucleic acids and total protein levels were significantly reduced in HCD alone fed group, quite the reverse, TC and TG levels increased. RT and/or AA supplementation along with HCD showed antilipidemic effects in hepatic cells while compared to only HCD group. Histopathological assessment of the liver sections revealed moderate degree of hepatotoxicity following HCD feedings and mild degree of hepatoxocity when combining either RT or AA with HCD, while HCD+RT+AA group had no such changes. The present data demonstrated that the degree of HCD induced hepatotoxicity is in positive correlation with hepatic lipids accumulations. Both RT and AA could reduce this toxicity through their antilipidaemic properties, which may be augmented by their combined intake.
Keywords:
Ascorbic acid, fatty liver, high cholesterol diet, hypercholesterolemia, rutin,
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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ISSN (Online): 2044-2467
ISSN (Print): 2044-2459 |
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